Method for modulating the efficiency of double-strand break-induced mutagenesis

摘要

A method for modulating double-strand break-induced mutagenesis at a genomic locus of interest in a cell, thereby giving new tools for genome engineering, including therapeutic applications and cell line engineering. A method for modulating double-strand break-induced mutagenesis, concerns the identification of effectors that modulate double-strand break-induced mutagenesis by use of interfering agents; these agents are capable of modulating double-strand break-induced mutagenesis through their respective direct or indirect actions on said effectors. Methods of using these effectors, interfering agents and derivatives, respectively, by introducing them into a cell in order to modulate and more particularly to increase double-strand break-induced mutagenesis. Specific derivatives of identified effectors and interfering agents, vectors encoding them, compositions and kits comprising such derivatives for modulating or increasing double-strand break-induced mutagenesis.

主权项

1. A method for increasing double-strand break-induced mutagenesis in a cell comprising introducing into said cell:
i. at least one interfering agent, wherein said at least one interfering agent targets the sequence of SEQ ID NO: 106; ii. at least one delivery vector comprising at least one double-strand break creating agent; thereby obtaining a eukaryotic cell in which double-strand break-induced mutagenesis is increased.