TEST METHOD FOR EFFICACY OF SILVER SURFACES AS A SANITIZER

摘要

A method that includes the steps: inoculating nutrient agar with bacterial stock to form a culture; incubating the culture to form a first incubated culture; incubating a portion of the first culture with nutrient agar to form a second culture; incubating a portion of the second culture to form a third culture; incubating the third culture to form an inoculated test plate; forming an inoculum by suspending bacteria from the inoculated test plate in a buffered test solution, adjusting the pH to ˜7 to 8, and adding organic soil at a concentration of approximately 10% to 30% by weight; inoculating a silver-containing surface region of a test carrier with a portion of the inoculum; incubating the inoculated test carrier; washing the test carrier in a neutralizing solution to form a residual test inoculum; and calculating the percent reduction in the number of surviving bacterial colonies in the residual test inoculum.

主权项

1. A method for testing the anti-microbial efficacy of a silver-containing surface region, comprising the steps:
inoculating nutrient agar with a portion of a stock having a plurality of bacterial organisms to form a culture; incubating the culture to form a first incubated culture; incubating a portion of the first incubated culture with nutrient agar to form a second incubated culture; incubating a portion of the second incubated culture with nutrient agar to form a third incubated culture; incubating the third incubated culture for approximately 48 hours to form an inoculated test plate with a plurality of bacterial colonies; forming an inoculum by suspending a portion of the plurality of bacterial colonies in a buffered test solution, adjusting the test solution to a pH of approximately 7 to 8, and adding an organic soil serum at a concentration of approximately 10% to 30% by weight to the test solution; inoculating a silver-containing surface region of a test carrier with a portion of the inoculum; incubating the inoculated test carrier for at least approximately two hours; washing the incubated and inoculated test carrier in a neutralizing solution to form a residual test inoculum; counting the number of surviving bacterial colonies per volume in the residual test inoculum; and calculating the percent reduction in the number of surviving bacterial colonies in the residual test inoculum relative to a residual control inoculum.