发明名称 |
ARTIFICIAL OOCYTE ACTIVATION |
摘要 |
The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca2+ in the oocyte prior to reduction of the intracellular Zn2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods. |
申请公布号 |
US2015150153(A1) |
申请公布日期 |
2015.05.28 |
申请号 |
US201414554504 |
申请日期 |
2014.11.26 |
申请人 |
Lee Kiho;Prather Randall S. |
发明人 |
Lee Kiho;Prather Randall S. |
分类号 |
C12N5/075;C12N15/877;A01K67/027 |
主分类号 |
C12N5/075 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method of activating an unfertilized livestock or domestic mammalian oocyte comprising decreasing intracellular Zn2+ concentration of the oocyte by contacting the oocyte with a Zn2+ binding moiety. |
地址 |
Columbia MO US |