发明名称 CHROMATOGRAPHIC PURIFICATION OF POLYNUCLEOTIDES WITH NEGATIVELY CHARGED PARTICLES
摘要 A method of purifying a sample that includes a polynucleotide includes the steps of (i) providing a packed chromatographic column having negatively charged porous particles, (ii) equilibrating the column to the conditions to which the polynucleotide in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the negatively charged porous particles within the packed chromatographic column, (iv) eluting the polynucleotide from the packed chromatographic column, where the polynucleotide is in a purer state and in the conditions to which the packed chromatographic column was equilibrated.
申请公布号 US2015148528(A1) 申请公布日期 2015.05.28
申请号 US201414555257 申请日期 2014.11.26
申请人 Agency for Science, Technology and Research 发明人 Gagnon Peter
分类号 C07H21/04 主分类号 C07H21/04
代理机构 代理人
主权项 1. A method of purifying a sample comprising a polynucleotide, the method comprising the steps of (i) providing a packed chromatographic column comprising negatively charged porous particles, (ii) equilibrating the packed chromatographic column to the conditions to allow the polynucleotide to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied is less than or equal to the interparticle space of the negatively charged porous particles within the packed chromatographic column, (iv) eluting the polynucleotide from the packed chromatographic column, wherein the polynucleotide is in a purer state.
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