发明名称 |
ANTIBODY AND APTAMER ENSEMBLE FOR CELL ISOLATION AND ENRICHMENT |
摘要 |
The subject invention pertains to devices and methods of isolating target cells from a population of cells. The devices comprise of microfluidic channels and aptamers and antibodies attached to the inner surface of the microfluidic channels, wherein the aptamers and the antibodies are capable of specific binding to one or more biomolecules present on the surface of the target cell. The methods of the current invention comprise passing the population of cells through the microfluidic channels to facilitate interaction and capture of the target cells by the aptamers and the antibodies attached to the inner surface of the microfluidic channels, washing the microfluidic channels by a washing solution to remove the cells non-specifically bound to the aptamers and the antibodies attached to the inner surface of the microfluidic channels, releasing the captured target cells from the microfluidic channels, and collecting the released target cells. |
申请公布号 |
WO2015077441(A2) |
申请公布日期 |
2015.05.28 |
申请号 |
WO2014US66590 |
申请日期 |
2014.11.20 |
申请人 |
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED |
发明人 |
FAN, ZHONGHUI, HUGH;ZHANG, JINLING |
分类号 |
G01N33/555;G01N1/14 |
主分类号 |
G01N33/555 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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