| 发明名称 |
PRODUCTION AND PURIFICATION OF ACTIVE EUKARYOTIC FORMYLGLYCINEGENERATING ENZYME (FGE) VARIANTS |
| 摘要 |
The invention features compositions and methods for generation and uses of formylglycine generating enzyme (FGE) variants. |
| 申请公布号 |
US2015141617(A1) |
申请公布日期 |
2015.05.21 |
| 申请号 |
US201414490331 |
申请日期 |
2014.09.18 |
| 申请人 |
Universität Bielefeld ;Georg-August-Universität Göttingen Stifung Öffentl |
发明人 |
Dierks Thomas;Ennemann Eva Charlotte;Radhakrishnan Karthikeyan;Alam Safaraz;Schmidt Bernhard;Wachs Michaela |
| 分类号 |
C12N9/02;C12P21/00 |
主分类号 |
C12N9/02 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. Process for producing eukaryotic Cα-formylglycine Generating Enzyme (FGE) or a functional variant thereof having Cα-formylglycine generating activity or a fragment thereof, comprising:
(i) culturing an insect cell containing an isolated polynucleotide encoding the eukaryotic FGE enzyme or a functional variant or a fragment thereof in a medium under conditions permitting the expression of FGE or functional variant or a fragment thereof; (ii) obtaining the produced FGE polypeptide of step (i). |
| 地址 |
Bielefeld DE |
