发明名称 |
METHOD FOR THE PRODUCTION OF A LYSATE USED FOR CELL-FREE PROTEIN BIOSYNTHESES |
摘要 |
The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof |
申请公布号 |
US2015140603(A1) |
申请公布日期 |
2015.05.21 |
申请号 |
US201414222937 |
申请日期 |
2014.03.24 |
申请人 |
RINA-NETZWERK RNA TECHNOLOGIEN GMBH |
发明人 |
Gerrits Michael;Strey Jan;Stiege Wolfgang |
分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
1. A method for the production of a lysate used for cell-free protein biosynthesis, comprising the following steps:
a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-free protein biosynthesis, is replaced by a foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the transformed organism according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is separated from the lysate obtained in step c) by means of a separation process that is selective for the marker sequence. |
地址 |
Berlin DE |