COMPOSITIONS FOR 2-METHYL-3-BUTEN-2-OL DEHYDRATION AND METHODS OF PRODUCING ISOPRENE USING SAME

摘要

The invention provides an immobilized enzyme system comprising a 2-methyl-3- buten-2-ol dehydratase enzyme immobilized on a support or a microorganism expressing a 2- methyl-3-buten-2-ol dehydratase enzyme immobilized on a support. The invention also provides methods of producing isoprene comprising the step of contacting 3-methyl-2-buten- 1- ol or 2-methyl-3-buten-2-ol with an immobilized enzyme system of the invention to effect the conversion of 3-methyl-2-buten-l-ol or 2-methyl-3-buten-2-ol to isoprene. In one embodiment the invention provides a method of producing isoprene by contacting 3-methyl- 2- buten-l-ol or 2-methyl-3-buten-2-ol with an optionally immobilized 2-methyl-3-buten-2-ol dehydratase to effect the conversion of 3-methyl-2-buten-l-ol or 2-methyl-3-buten-2-ol to isoprene and optinally collecting the isoprene.