LONGITUDINAL ASSAY

摘要

Embodiments of the invention relate generally to macro and small molecule detection and, more particularly, to methods for detecting macro and small molecules, including bio-molecules, in a liquid or gaseous sample. Methods according to embodiments of the invention are useful in the identification, discovery, and validation of biomarkers, as well as the screening of individuals for such biomarkers for diagnostic, therapeutic, and forensic purposes. In one embodiment, the invention provides a method of detecting an analyte in a fluid sample, the method comprising: passing a fluid sample containing a labeled analyte across at least one assay surface containing a capture agent for the analyte; detecting the labeled analyte; repeating the passing and detecting steps at least once; and creating a binding curve for the analyte based on the detecting of the labeled analyte.

主权项

1. A method of distinguishing specific binding of an analyte and capture agent from non-specific binding of non-analyte molecules to the capture agent, the method comprising:
obtaining a binding curve for a capture agent to sample interaction (said interaction being between analyte and/or non-analyte molecules within the sample) based on a plurality of temporally-spaced detections of the capture agent to sample interactions within an assay; determining a first detection intensity of the capture agent to sample interactions and a first temporal point in the binding curve at which a rise to an equilibrium may be inferred; and attributing to non-specific binding a first portion of at least one detected intensity subsequent to the first temporal point that is equal to the first detection intensity.