Method and apparatus for stain separation using vector analysis

摘要

A computer-implemented method and apparatus for stain separation of a pathology image using stain vector analysis comprising converting an original image into an optical domain image, performing stain vector analysis on the optical domain image to obtain one or more stain vectors, deconvoluting the vectors adaptively to produce one or more separated stain images.

主权项

1. A computer-implemented method for stain separation of a pathology image using stain vector analysis comprising:
converting an original image into an optical domain image; performing stain vector analysis on the optical domain image to obtain one or more stain vectors; deconvoluting the one or more stain vectors to produce one or more separated stain images; isolating stain amounts in each color channel of the one or more separated stain images; comparing, for each pixel in the original image, a pixel value from one of the one or more stain images to a pixel value in another image of the one or more stain images; and selecting, for each pixel in the original image, a pixel value with a largest stain value, wherein a plurality of selected pixel values forms a strongest stain image.