SINGLE-CHAIN ANTIBODIES AND OTHER HETEROMULTIMERS

摘要

The invention provides engineered heteromultimeric protein complexes constructed using one, two, or three tethers and methods for making, using, and purifying such complexes, such as antibodies with different binding properties.

主权项

1. A single-chain antibody comprising a single polypeptide comprising the following components:
(a) a first VH (VH1) domain and a second VH (VH2) domain, (b) a heterodimerization (HD) tether; and (c) one or more heavy chain constant domains,wherein said one or more heavy chain constant domains are selected from a first CH2 (CH21) domain, a first CH3 (CH31) domain, a second CH2 (CH22) domain, and a second CH3 (CH32) domain; and wherein said components are positioned relative to each other in an N-terminal to C-terminal direction as follows: VH1-optional CH21-optional CH31-HD tether-VH2-optional CH22-optional CH32.