RECOMBINANT DEAMIDATED GLIADIN ANTIGEN

摘要

PROBLEM TO BE SOLVED: To provide a method for determining whether a subject is suffering from celiac disease by making a sample of bodily fluid from the subject contact with an antigen formed from a gliadin fusion protein immobilized on a solid support; and to provide the antigen for detecting the celiac disease.SOLUTION: An antigen for detecting celiac disease comprises a recombinant deamidated gliadin covalently linked to a tag to form a gliadin fusion protein. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.