SELECTION OF NUCLEIC ACIDS BY SOLUTION HYBRIDIZATION TO OLIGONUCLEOTIDE BAITS

摘要

Methods of selection of nucleic acids using solution hybridization, methods of sequencing nucleic acids including such selection methods, and products for use in the methods are disclosed.

主权项

1. A method for solution-based selection of nucleic acids comprising
hybridizing in solution (1) a group of nucleic acids and (2) a set of bait sequences, to form a hybridization mixture, contacting the hybridization mixture with a molecule or particle that binds to or is capable of separating the set of bait sequences from the hybridization mixture, and separating the set of bait sequences from the hybridization mixture to isolate a subgroup of nucleic acids that hybridize to the bait sequences from the group of nucleic acids, wherein the subgroup of nucleic acids is a part or all of a set of target sequences that is desired to be selected, wherein the sequence composition of the set of bait sequences determines the nucleic acids directly selected from the group of nucleic acids, and wherein the bait sequences are oligonucleotides between about 70 nucleotides and 1000 nucleotides in length.