| 主权项 |
1. A Pleuropterus multiflorus extract for promoting the secretion of an insulin-like growth factor and bone structure growth, the method of its manufacture comprising:
(a) mixing from 6 to 10 volumes of water relative to the dry weight of Pleuropterus multiflorus, having a temperature ranging from 80 to 100° C., with Pleuropterus multiflorus, extracting while stirring the mixture for from 6 to 10 hours, followed by cooling the extract to 70° C.; (b) increasing the content of Pleuropterus multiflorus-derived glycoside by adding 4 to 5 wt % of α-amylase relative to the dry weight of Pleuropterus multiflorus to the Pleuropterus multiflorus extract, and fermenting the mixture at from 70 to 80° C. for from 2 to 3 hours; (c) upon completion of the fermentation, terminating the activity of α-amylase by increasing the temperature of the fermented Pleuropterus multiflorus extract to from 95 to 99° C., followed by quick freezing; (d) performing a first purification for removing insoluble microparticles and proteins from the Pleuropterus multiflorus extract by separating solids from the Pleuropterus multiflorus extract using a centrifugal hydro-extractor or a filter press, and subjecting them to ultracentrifugation at a rate ranging from 15,000 to 20,000 rpm; (e) performing a second purification for removing fat components, inactive organic compounds, tannin components, and heavy metals from by passing the Pleuropterus multiflorus extract, which has been purified by the first purification, through an activated carbon filter; (f) performing a third purification for removing high molecular carbohydrates and microorganisms from the Pleuropterus multiflorus extract, which has been purified by the second purification, which includes separating a retentate and a permeate from the Pleuropterus multiflorus extract via a Tangential Flow Filtration (TFF) type micro or ultra filtration system having a 0.2 to 0.45 μm thick membrane, wherein the permeate is recovered therefrom but, when the flow rate drastically decreases due to a substantial increase in the filtration pressure caused by the increase in the retentate concentration, the retentate is diluted with purified water and repeatedly filtered from 2 to 9 times; (g) concentrating the purified Pleuropterus multiflorus extract by adding it into a rotary evaporator and reducing the pressure at a temperature ranging from 40 to 60° C. until the brix reaches 10 or higher; and (h) upon completion of concentration, further comprising adding the concentrated Pleuropterus multiflorus extract into a lyophilizer and subjecting it to a stepwise temperature increased lyophilization cycle at a temperature ranging from −60 to 40° C. for from 30 to 40 hours, thereby obtaining a powdered Pleuropterus multiflorus extract, wherein the Pleuropterus multiflorus extract is capable of promoting the secretion of insulin-like growth factor and bone structure growth; and comprises a Pleuropterus multiflorus-derived saponin glycoside as an active ingredient. |
