发明名称 |
ULTRASOUND MEDIATED DELIVERY OF SUBSTANCES TO ALGAE |
摘要 |
Embodiments provided herein generally relate to methods and materials for ultrasound-mediated introduction of exogenous substances into microorganisms. Some embodiments relate to methods of introducing an exogenous material into a microorganism such as an algae. The methods can include, for example, providing a microorganism (e.g., algae); providing a population of bubbles that comprise one or more nucleic acid molecules and/or one or more polypeptide or one or more protein molecules; contacting the population of bubbles with the algae; applying ultrasound to the bubbles and algae with sufficient energy to cavitate one or more of bubbles comprising the one or more nucleic acid molecules in proximity to the algae; and maintaining the algae and the one or more burst bubbles comprising the one or more nucleic acid molecules in contact for a period of time sufficient to permit entry of at least one nucleic acid molecule into the algae. |
申请公布号 |
US2015125960(A1) |
申请公布日期 |
2015.05.07 |
申请号 |
US201314400136 |
申请日期 |
2013.05.11 |
申请人 |
University of Hawaii |
发明人 |
Liaw Eric;Walton Chad B.;Shohet Ralph V. |
分类号 |
C12N15/82 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
1. A method of introducing an exogenous material into an algae, comprising:
providing an algae; providing a population of bubbles that comprise one or more nucleic acid molecules and/or one or more polypeptide or protein molecules; contacting the population of bubbles with the algae; applying ultrasound to the bubbles and algae with sufficient energy to cavitate one or more of bubbles comprising the one or more nucleic acid molecules in proximity to the algae; and maintaining the algae and the one or more burst bubbles comprising the one or more nucleic acid molecules in contact for a period of time sufficient to permit entry of at least one nucleic acid molecule into the algae. |
地址 |
Honolulu HI US |