Method and arrays for detecting biological molecules

摘要

Arrays and methods for detecting one or more biological molecules, where the methods generally comprise the steps of: providing a first support structure fixed with one or more reagents; providing a second support structure fixed with a plurality of ligands; contacting the reagents fixed to the first support structure with the ligands fixed to the second support structure whereby a plurality of the reagents bind to one or more of the ligands; and separating the first support structure from the second support structure so that the plurality of the bound reagents remain bound to a plurality of ligands on the second support after separation. In one preferred method, proteins are immobilized on a support with adequate strength so that the proteins can be dissociated from the support under certain conditions, such as after binding with other proteins immobilized on another support. For example, antibody arrays produced according to the present invention may be used to detect protein expressions in a protein lysate and may be used in immunostaining to reveal the presence and location of protein in cells.

主权项

1. A method for detecting one or more ligands, comprising the steps of
providing a first support to which one or more reagents are immobilized, each at one or more predetermined positions to make a reagent array; separating the ligands in a gel by gel electrophoresis; contacting the gel with a second support; transferring the ligands from the gel to the second support, whereby the ligands are immobilized on the second support; contacting the reagents immobilized on the first support with the ligands immobilized on the second support whereby a plurality of the reagents bind to one or more of the ligands; separating the first support from the second support; and detecting a plurality of reagents bound to the ligands on said second support, whereby one or more ligands are detected.