CELL CULTURE SYSTEM AND CELL CULTURE METHOD

摘要

A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.

主权项

1. A cell culture system comprising:
a cell culture vessel for culturing cells; a component controlling fluid storage vessel; a culture fluid component controller having an inlet and an outlet for a cell culture fluid and/or component controlling fluid and comprising a semipermeable membrane; an inlet-connected fluid feeding circuit from the cell culture vessel and/or the component controlling fluid storage vessel to an inlet of the culture fluid component controller; an outlet-connected fluid feeding circuit from the cell culture vessel and/or the component controlling fluid storage vessel to an outlet of the culture fluid component controller; a perfuser which perfuses the cell culture fluid and/or the component controlling fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid component controller; and a controller which controls the amount of fluid in the cell culture vessel, wherein components of the cell culture fluid in the cell culture vessel and components of the component controlling fluid in the component controlling fluid storage vessel can be controlled by the culture fluid component controller in a continuous manner; and at the same time, the amount of cell culture fluid in the cell culture vessel can be controlled to be substantially constant.