| 摘要 |
A method of measuring gene abundance is provided, which includes the steps of: amplifying, in one reaction solution, nucleic acids encoding at least two genes whose abundances in nucleic acids contained in a subject sample may be different using a first primer set, which includes at least two types of first primers each of which is capable of introducing a single additional base sequence to the resulting amplification product and corresponds to each of the at least two genes, and a second primer for amplifying a nucleic acid containing the single additional base sequence, thereby obtaining at least two types of amplification products each of which contains the single additional base sequence and corresponds to each of the at least two genes; and detecting signals that are emitted based on the abundances of the at least two types of amplification products to determine the abundances of the at least two genes based on the thus detected signals. |
