Microchip for analyzing nucleic acid and method of nucleic acid analysis using the same

摘要

A method for nucleic acid analysis including injecting a slight amount of nucleic acid sample for analysis, characterized in that most of a nucleic acid sample which is not used, excluding the slight amount of the nucleic acid sample which is used for analysis, can be obtained as a pure product which is not contaminated with a fluorescent material, and a microchip for analyzing nucleic acid which enables such method for nucleic acid analysis, are provided. The method for nucleic acid analysis may analyze at least two different nucleic acid samples in a continuous manner by sequentially injecting the at least two different nucleic acid samples.

主权项

1. A method for nucleic acid analysis, using a microchip comprising a nucleic acid sample channel part comprising a nucleic acid sample supply part and a nucleic acid sample discharge part, a standard sample channel part comprising a standard sample supply part and a standard sample discharge part, a separation channel part comprising a fluorescent dye storage part and a fluorescent discharge part at its both ends, a nucleic acid sample separation supplementary channel connecting the nucleic acid sample channel part and the separation channel part, and a standard sample separation supplementary channel connecting the standard sample channel part and the separation channel part, wherein the channels except the nucleic acid sample channel part and the standard sample channel part are filled with a polymer gel, comprising:
(1) supplying a nucleic acid sample through the nucleic acid sample supply part, so that the supplied nucleic acid sample flows toward the nucleic acid sample discharge part; (2) supplying a standard size nucleic acid as a standard sample through the standard sample supply part; (3) injecting a slight volume of the nucleic acid sample which flows in the nucleic acid sample channel part into the separation channel part through the nucleic acid sample separation supplementary channel by applying a negative voltage for 0.1 to 2 seconds to an end of a nucleic acid sample injection supplementary channel that is extended from a connection part which is a point at which the nucleic acid sample channel part and the nucleic acid sample separation supplementary channel are connected; (4) injecting the standard sample into the separation channel part through the standard sample separation supplementary channel by applying a negative voltage for 0.1 to 2 seconds to an end of a standard sample injection supplementary channel extended from a connection part which is a point at which the standard sample channel part and the standard sample separation supplementary channel are connected; (5) reacting the fluorescent dye with the nucleic acid sample and the standard sample by applying a negative voltage to a fluorescent sample discharge part of the separation channel part, allowing a fluorescent dye having a positive charge to transfer to the fluorescent sample discharge part and to transfer the nucleic acid sample and the standard sample injected into the separation channel part in a direction opposite to the fluorescent sample discharge part; and (6) separating the nucleic acid sample and the standard sample reacted with the fluorescent dye in the separation channel part and conducting fluorescence detection wherein the slight volume of the nucleic acid sample is in the range of 0.1 pL to 10 nL.