Localized surface plasmon resonance based super resolved total internal reflection fluorescence imaging apparatus, and detection module therefor

摘要

A total internal reflection fluorescence imaging apparatus according to an embodiment of the invention includes: a metal nanostructure layer, which includes a metal thin film and a nanostructure formed over the metal thin film; a light source unit, which provides incident light such that the incident light is totally reflected off the metal nanostructure layer and an evanescent wave localized in a horizontal direction is created between the metal nanostructure layer and a specimen arranged over the metal nanostructure layer; and a fluorescence image extracting unit, which extracts and images a fluorescence signal generated by the specimen due to the evanescent wave localized in a horizontal direction.

主权项

1. A total internal reflection fluorescence imaging apparatus, comprising:
a metal nanostructure layer including a metal thin film and a nanostructure formed on the metal thin film; a light source unit configured to provide incident light such that the incident light is totally reflected off the metal nanostructure layer and an evanescent wave localized in a horizontal direction of the light source unit is created between the metal nanostructure layer and a biological specimen arranged on the metal nanostructure layer; and a fluorescence image extracting unit configured to extract and image a fluorescence signal generated by the biological specimen due to the evanescent wave localized in a horizontal direction of the fluorescence image extracting unit, wherein the metal nanostructure layer further comprises a metal grid formed over the metal thin film, and a plurality of nanostructures are formed over the metal grid.