Means and methods for producing authentic human basic fibroblast growth factor

摘要

The present invention is concerned with an engineered biological system for production of authentic human basic fibroblast growth factor (hbFGF). The system makes use of a bacterial host and has a recombinant DNA construct with an insert. The insert contains a first VegC promoter, lacq operator, a second VegC promoter and DNA coding for human basic fibroblast growth factor.

主权项

1. A method for production of authentic human basic fibroblast growth factor (hbFGF) protein by a host, wherein the authentic human basic fibroblast growth factor (hbFGF) has a sequence consisting of SEQ ID NO:37, said method comprising:
a) providing Bacillus subtilis as the host; b) preparing a recombinant DNA construct with an insert including a first VegC promoter, lacq operator, a second VegC promoter and DNA coding for said authentic human basic fibroblast growth factor; c) introducing said DNA construct in the host for expression of said authentic human basic fibroblast growth factor protein; d) allowing said Bacillus subtilis to grow in a cell culture and express said authentic human basic fibroblast growth factor protein; e) allowing said Bacillus subtilis to secrete said authentic human basic fibroblast growth factor protein in the cell culture; and f) collecting said secreted authentic human basic fibroblast growth factor protein from the cell culture.