| 摘要 |
PROBLEM TO BE SOLVED: To provide cells useful for immuno-based botulinum toxin serotype A activity assays.SOLUTION: An established clonal cell line comprising cells susceptible to intoxication by BoNT/A, in which the cells exhibit at least a 1.5-fold differentiation in the gene expression levels of more than three genes as compared to the expression levels of the genes in cells derived from the parental SiMa cell line DSMZ ACC 164; the gene expression levels are selected from a group consisting of ADAMTS9, ASCL1, BASP1, DOK5, DPYD, GNB4, GNG11, GTSF1, MAOA, MINA, MSN, PEG3, PLK2, PRSS12, RNF182, SLC44A5, SPARC, TFPI2, and TPTE; and the clonal cell line comprises cells susceptible to BoNT/A intoxication by about 100 pM or less of a BoNT/A. |
