PROTEASOME INHIBITION ASSAY AND METHODS OF USE

摘要

Assays that can measure the effect of proteasome inhibitors on target cells in a biological sample are provided. The assays include detecting proteasome activity in a target cell comprising: a) contacting a biological sample comprising one of more target cells with an 1KB degradation agonist to activate the one or more target cells in the biological sample; b) contacting the activated biological sample with a first labeled binding agent that is capable of binding ΙκΒ; c) detecting a target cell in the biological sample of b); and d) determining an amount of the first labeled binding agent bound to the detected target cell, thereby detecting proteasome activity in the target cell.