ENZYMES AND METHODS FOR CLEAVING N-GLYCANS FROM GLYCOPROTEINS

摘要

Provided herein are deglycosylating enzymes that remove a broad range of N-glycans from N-glycosylated proteins. Further provided are methods of recombinantly producing and expressing the deglycosylating enzymes. The presently described deglycosylating enzymes can be used to produce free glycans for characterization, and for prebiotic and immunostimulatory uses. In addition, the presently described deglycosylating enzymes can be used to produce deglycosylated proteins for characterization, to improve digestion, and to reduce immunogenicity.

主权项

1. An recombinant polypeptide comprising a sequence of: GLDIDME (SEQ ID NO:1),
wherein the polypeptide can cleave high mannose, complex, and hybrid N-glycans from a glycoprotein, and wherein the polypeptide lacks a transmembrane domain that spans a cell membrane.