摘要 |
The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base. |
申请人 |
UNIVERSITY OF IOWA RESEARCH FOUNDATION;INTEGRATED DNA TECHNOLOGIES, INC.;MCNAMARA, JAMES, O., II;STOCKDALE, KATIE, R.;HUANG, LINGYAN;HORSWILL, ALEXANDER, R.;BEHLKE, MARK, A.;HERNANDEZ, FRANK, J. |
发明人 |
MCNAMARA, JAMES, O., II;FLENKER, KATIE, R.;HUANG, LINGYAN;HORSWILL, ALEXANDER, R.;BEHLKE, MARK, A.;HERNANDEZ, FRANK, J. |