Thrombin isolated from blood and blood fractions

摘要

Methods, apparatus, and compositions related to generating and using thrombin. Methods include preparing a solution comprising thrombin by precipitating fibrinogen from a liquid comprising whole blood or a blood fraction. Precipitated fibrinogen is removed from the liquid to form a post-precipitation liquid that is incubated with calcium and a plurality of beads to form a clot. A solution comprising thrombin is separated from the clot. Thrombin prepared thereby can be used as a tissue sealant and in methods of applying a tissue sealant to subject, including application of an autologous tissue sealant.

主权项

1. A method of preparing a solution comprising thrombin, the method comprising:
precipitating fibrinogen from a liquid comprising platelet-poor plasma or whole blood; removing precipitated fibrinogen from the liquid by centrifugation or filtration to form a post-precipitation liquid; incubating the post-precipitation liquid with calcium and a plurality of beads to activate a portion of the post-precipitation liquid to generate a solution comprising thrombin; and separating the solution comprising thrombin from the beads.