| 发明名称 | Production of hemagglutinin-neuraminidase protein in microalgae | ||
| 摘要 | The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof. | ||
| 申请公布号 | US9012197(B2) | 申请公布日期 | 2015.04.21 |
| 申请号 | US201012980320 | 申请日期 | 2010.12.28 |
| 申请人 | Merial, Inc.;Sanofi Vaccine Technologies, S.A.S. | 发明人 | Bayne Anne-Cécile V.;Lippmeier James Casey;Apt Kirk Emil;Guo Xuan;Pritchard Joyce A. |
| 分类号 | C12N9/26;C12N9/14;C12N1/12;C12P21/06;C07H21/04;C07K1/00;C12N7/00;C12N15/82;C12N9/24 | 主分类号 | C12N9/26 |
| 代理机构 | Merial, Inc. | 代理人 | Jarecki-Black Judy;Chen Ruoying;Merial, Inc. |
| 主权项 | 1. A method for production of a hemagglutinin-neuraminidase (HN) polypeptide, wherein said HN polypeptide is glycosylated and comprises full length HN polypeptide, a membrane domain and a native HN signal peptide, said method comprising culturing a recombinant microalgal host cell in a medium, wherein the recombinant microalgal host cell comprises a heterologous nucleic acid molecule comprising the sequence of SEQ ID NO:1 encoding the HN polypeptide to produce the HN polypeptide, and wherein the microalgal host cell is free of polynucleotide sequences encoding other heterologous viral polypeptides, whereby the HN polypeptide is secreted into the medium by action of the signal peptide, and recovering the HN polypeptide from the medium. | ||
| 地址 | Duluth GA US | ||