Assays for detection of glycosaminoglycans

摘要

Disclosed herein are novel methods, assays and kits useful for the diagnosis and monitoring of subjects with mucopolysaccharidoses (MPS), The methods, assays and kits are particularly useful for detecting the presence of one or more glycosaminoglycans which correlate to MPS and its severity in a variety of biological samples.

主权项

1. A method for determining the concentration of one or both of dermatan sulfate (DS) and heparin sulfate (HS) in a sample, the method comprising (a) treating a sample with at least one of chondroitinase B and one or more heparineses, (b) combining a serine protease shown in FIG. 7 or 8 a labeled substrate for said serine protease, an inhibitor of said serine protease, and the sample under conditions and for a time suitable for cleavage of the labeled substrate by the serine protease to produce a detectable signal, (c) detecting the detectable signal, and (d) comparing the amount of detectable signal with a standard to determine the concentration of said one or both of DS and HS in said sample.