COMPOSITE VISIBLE COLORANT AND METHOD FOR QUANTITATIVE AMPLIFICATION

摘要

Methods and compositions for using colorants with real-time amplification reactions and normalization passive dyes are provided.

主权项

1. A composite colorant formulation comprising:
a visually-detectable dye; and an internal reference molecule comprising a first fluorophore, which first fluorophore upon excitation emits energy at a specific wavelength range; wherein: the visually-detectable dye partially absorbs energy at the specific wavelength range; and the formulation does not comprise sufficient components for target nucleic acid amplification if added to a target nucleic acid and primers.