Luminescence quenching compounds

摘要

The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.

主权项

1. A method of labeling a peptide, nucleotide, steroid, drug, amino acid, protein, nucleic acid polymer, carbohydrate, or lipid, wherein the method involves conjugation of the peptide, nucleotide, steroid, drug, amino acid, protein, nucleic acid polymer, carbohydrate, or lipid with a compound having the following structure: wherein R1 to R3, R6, R8 to R11 and R13 to R16 are hydrogen, alkyl having from 1-20 carbons, alkoxy having from 1-20 carbons, trifluoromethyl, halogen, methylthio, sulfonyl, carbonyl, hydroxyl, amino, thiol, or nitro, and wherein R4 is a chemically reactive group, and wherein R5 is an alkyl having from 1-20 carbons, and wherein R7 is a thiophene or a substituted thiophene, and wherein the conjugation comprises covalent attachment of the compound to a functional group on the peptide, nucleotide, steroid, drug, amino acid, protein, nucleic acid polymer, carbohydrate, or lipid, using the chemically reactive group.