| 发明名称 |
Acceptor framework for CDR grafting |
| 摘要 |
The present invention relates to an antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using a particularly well suited antibody acceptor framework. Antibodies generated by the methods of the invention are useful in a variety of diagnostic and therapeutic applications. |
| 申请公布号 |
US9005924(B2) |
申请公布日期 |
2015.04.14 |
| 申请号 |
US201313765302 |
申请日期 |
2013.02.12 |
| 申请人 |
ESBATech |
发明人 |
Escher Dominik |
| 分类号 |
C12P21/04;C07K16/18;C07K16/46 |
主分类号 |
C12P21/04 |
| 代理机构 |
|
代理人 |
Derry Jason J. |
| 主权项 |
1. A method of humanizing a rabbit immunobinder, the method comprising:
(a) grafting the CDR H1, CDR H2 and CDR H3 sequences from a donor rabbit immunobinder into the human heavy chain acceptor framework comprising SEQ ID NO: 1; and (b) grafting the CDR L1, CDR L2 and CDR L3 sequences from a donor rabbit immunobinder into a human light chain acceptor framework into a light chain acceptor framework that has at least 85% identity to SEQ ID NO: 2. |
| 地址 |
CH |
