Regulation of hematopoietic stem cell functions through microRNAs

摘要

The present disclosure relates to regulation of functions of hematopoietic stem cells (HSCs) by delivering of miRNAs, including miR-125b, miR-126, and miR-155, to HSCs. For example, in some embodiments, blood output in a mammal can be increased by administering miR-125b, miR-126, and/or miR-155 oligonucleotides. Also disclosed are methods for promoting hematopoietic stem cell engraftment and method for treating a myeloproliferative disorder.

主权项

1. A method for increasing blood output in a mammal, comprising:
providing a mammal in need of increasing blood output; and administering an oligonucleotide comprising a microRNA-125b (miR-125b) sequence to hematopoietic stem cells (HSCs) in the mammal, thereby increased blood output in the mammal, wherein the miR-125b sequence comprises a mature miR-125b1 sequence, a mature miR-125b2 sequence, a pre-miR-125b1 sequence, or a pre-miR-125b2 sequence.