Dual cofactor specific ribitol dehydrogenase and use thereof

摘要

There are provided a novel ribitol dehydrogenase, a residue determining double coenzyme specificity, and a method for preparing L-ribulose using the same, and more particularly, to a ribitol dehydrogenase producing rare sugars, nucleic acid molecules encoding the same, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the ribitol dehydrogenase, and a method for preparing L-ribulose using the ribitol dehydrogenase. The ribitol dehydrogenase having double coenzyme specificity, which is derived from Zymomonas mobilis, can effectively be used for preparing high-priced rare sugars and an investigation of coenzyme specificity determinants for the ribitol dehydrogenase is applied for all of dedydrogenases as a based technique.

主权项

1. A ribitol dehydrogenase mutant, in which serine, an amino acid at position 156 of the ribitol dehydrogenase having an amino acid sequence as set forth in SEQ ID NO: 4 is substituted with aspartic acid.