| 摘要 |
<p>Provided herein is a computer-implemented method for quantifying the spatial distribution of cells or sub-cellular structures, the method comprising the following steps performed by a computer: (a) receiving for a subject image data comprising a plurality of biomarkers, N, wherein the data represents a spatial map of the biomarkers; (b) processing the data to obtain a set of coordinates, wherein each coordinate denotes the location of a cell or sub-cellular structure represented by a biomarker or combination of biomarkers; (c) processing the set of coordinates into a two-dimensional symmetric (2N- 1) x (2N-1) or 2N x 2N matrix (D), wherein each element (i, j) of the matrix indicates the frequency of a cell or sub-cellular structure represented by a first biomarker or combination of biomarkers (i) being observed within an interaction distance, d l , of a cell or sub-cellular structure represented by a second biomarker or combination of biomarkers (j), and wherein i and j may be the same or different; and (d) using a classifier to assign the subject to a group or category based on the two-dimensional symmetric matrix (D).</p> |
