发明名称 |
METHODS AND DEVICES FOR ELECTRICAL SAMPLE PREPARATION |
摘要 |
Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing. |
申请公布号 |
EP2737047(A4) |
申请公布日期 |
2015.04.08 |
申请号 |
EP20120816873 |
申请日期 |
2012.07.25 |
申请人 |
QVELLA CORPORATION |
发明人 |
TALEBPOUR, SAMAD;KHINE, AYE AYE;MAASKANT, ROBERT;ALAVIE, TINO |
分类号 |
C12M1/42;B81B1/00;B81B7/02;C12M1/33;C12N1/06;C12N13/00;C12N15/10;C12Q1/68 |
主分类号 |
C12M1/42 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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