发明名称 |
Process for the preparation of disinfected human cell suspensions |
摘要 |
In a process for preparing disinfected preparations from mammalian tissues, mammalian tissue is perfused at one step with an enzyme-free liquid antibiotic composition that includes at least one antibiotic and is contained in at least one perfusion buffer. In a step occurring after such one step, enzymatic antibiotic-free treatment of the tissue is carried out to obtain a single-cell suspension. In another step disinfected preparations are obtained. |
申请公布号 |
US8999710(B2) |
申请公布日期 |
2015.04.07 |
申请号 |
US201113816909 |
申请日期 |
2011.07.30 |
申请人 |
Cytonet GmbH & Co. KG |
发明人 |
Aleksandrova Krasimira;Barthold Marc;Arseniev Lubomir;Griesel Carsten;Priesner Christoph |
分类号 |
C12N5/071;A01N1/02 |
主分类号 |
C12N5/071 |
代理机构 |
Jordan and Hamburg LLP |
代理人 |
Jordan and Hamburg LLP |
主权项 |
1. A process for preparing disinfected, single-cell suspension preparations from mammalian tissue, comprising:
prior to any enzymatic treatment of said mammalian tissue, performing a primary decontamination of a surface of the mammalian tissue comprising rinsing the mammalian tissue to obtain a rinsed mammalian tissue; after said primary decontamination, perfusing said rinsed mammalian tissue with an enzyme-free liquid antibiotic composition that comprises at least one antibiotic and is contained in at least one perfusion buffer, wherein the enzyme-free liquid antibiotic composition is perfused through vascular tissue inherent to the mammalian tissue to obtain an antibiotic-treated mammalian tissue; subsequent to all antibiotic perfusion, carrying out an enzymatic antibiotic-free treatment of the tissue to obtain the disinfected, single-cell suspension preparations comprising contacting said antibiotic-treated mammalian tissue with collagenase I and clostridiopeptidase I to obtain disinfected single-cell suspension preparations; washing the obtained disinfected single-cell suspension preparations with an antibiotic-free washing buffer to obtain a washed disinfected single-cell suspension preparation; subsequent to said washing step, and without an intervening antibiotic treatment, performing a microbiological sterility test of the washed disinfected single-cell suspension preparation; and wherein said washing is adapted to either one of reduce or eliminate said antibiotic from said disinfected single-cell suspension so as not to have a negative effect on said microbiological sterility test. |
地址 |
Weinheim DE |