Production of canola protein isolate without heat treatment

摘要

The supernatant from the deposition of canola protein micellar mass is processed to provide a canola protein isolate which is soluble in an aqueous acidic environment.

主权项

1. A process of preparing a canola protein isolate having a protein content of at least 90 wt % (N×6.25) on a dry weight basis, which comprises:
adding a calcium salt to supernatant from the precipitation of a canola protein micellar mass to provide a conductivity of about 5 mS to about 30 mS to form a calcium phytate precipitate, removing precipitated calcium phytate from the resulting solution to provide a clear solution, adjusting the pH of the clear solution to about 2.0 to about 4.0, concentrating the pH-adjusted clear solution to a protein content of at least about 50 g/L to produce a clear concentrated canola protein solution, optionally diafiltering the clear concentrated canola protein solution, optionally effecting a colour removal step, and drying the concentrated protein solution.