METHOD FOR ASSESSING JUICE/CIDER QUALITY AND/OR SAFETY

摘要

A novel method for isolating DNA from juices and ciders is described. This method is low cost and yield large quantities of highly purified DNA even though one uses a small quantity of juice or cider. A method for determining if a juice or cider is safe to consume and/or the quality of the juice or cider are also described. For these methods, one can perform qPCR on the DNA which can be obtained using the disclosed method or any other prior art method, and comparing the amount of DNA from microorganisms is present in the juice and/or cider to determine the safety and/or quality of the juice and/or cider. These methods work even if the liquid was pasteurized.

主权项

1. An improved method for isolating nucleic acids from a liquid comprising:
separating solid components from said liquid; lysing the cells present in said solid components to release nucleic acids, proteins, polysaccharides, lipids, and non-polar material present in said cells; separating said proteins, said lipids, said polysaccharides, and said non-polar material from said nucleic acids.