| 发明名称 |
Chimeric enzymes with improved cellulase activities |
| 摘要 |
Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose. |
| 申请公布号 |
US8993276(B2) |
申请公布日期 |
2015.03.31 |
| 申请号 |
US201313940936 |
申请日期 |
2013.07.12 |
| 申请人 |
Alliance for Sustainable Energy, LLC |
发明人 |
Xu Qi;Baker John O.;Himmel Michael E. |
| 分类号 |
C12P19/14;C12N9/96;C12N9/14;C12N9/24;C12P21/06;C12P19/34;C12N15/00;C12N1/20;C07H21/04;C07K1/00;C12N9/42 |
主分类号 |
C12P19/14 |
| 代理机构 |
|
代理人 |
Stolpa John C. |
| 主权项 |
1. An isolated nucleic acid molecule encoding a chimeric CbhA polypeptide comprising domains from Clostridium thermocellum cellobiohydrolase A (CbhA) and Caldicellulosiruptor bescii cellobiohydrolase A (CelA) polypeptides, wherein the chimeric CbhA polypeptide has an amino acid sequence at least 95% identical to “SEQ ID NO:6,” and wherein the chimeric CbhA polypeptide has a cellulase activity at least 1.5-fold greater than the wild-type CbhA polypeptide. |
| 地址 |
Golden CO US |
