Modified xylan production

摘要

Methods and means for producing xylan structures in plants having a non-native saccharide moiety substitution side chain component, plants and plant cells comprising modified xylan structures, methods of identifying mutant plants comprising xylan structures in plants having a non-native saccharide moiety substitution pattern side chain component, uses thereof, and isolated xylan structures and uses thereof.

主权项

1. A transformed plant cell comprising a xylan structure having a non-native [Me]GlcA substitution pattern side chain component,
wherein said transformed plant cell comprises an introduced DNA sequence encoding an antisense RNA molecule operably linked to a promoter and a terminator, said promoter and terminator being capable of functioning in a plant cell, wherein said antisense RNA molecule is complementary to a portion of the coding sequence for a xylan glucuronyl transferase (XGAT); or, wherein said transformed plant cell comprises an introduced DNA sequence encoding a sense RNA molecule operably linked to a promoter and a terminator, said promoter and terminator being capable of functioning in a plant cell, wherein said sense RNA molecule is a coding sequence for a xylan glucuronyl transferase (XGAT), and wherein the xylan glucuronyl transferase (XGAT) is a protein selected from the group At4g33330 (SEQ ID NO:12), At3g18660 (SEQ ID NO:13), At1g77130 (SEQ ID NO:14), At1g08990 (SEQ ID NO:15), At1g54940(SEQ ID NO:16), PttGT8A (SEQ ID NO:17), PttGT8B (SEQ ID NO:18), PttGT8C ((SEQ ID NO:19), CAK29728 ((SEQ ID NO:20), Os03g0184300 (SEQ ID NO:21), Os01g0880200 (SEQ ID NO:22), Os05g0426400 (SEQ ID NO:23), Os1 010047 (SEQ ID NO:24), AAK92624 (SEQ ID NO:25), and ABE88903 ((SEQ ID NO:28) or fragments thereof or a protein encoded by a nucleotide sequence comprising AK250038 (SEQ ID NO:26) or AY110752 (SEQ ID NO:27).