Protocells and their use for targeted delivery of multicomponent cargos to cancer cells

摘要

Various embodiments provide materials and methods for synthesizing protocells for use in targeted delivery of cargo components to cancer cells. In one embodiment, the lipid bilayer can be fused to the porous particle core to form a protocell. The lipid bilayer can be modified with targeting ligands or other ligands to achieve targeted delivery of cargo components that are loaded within the protocell to a target cell, e.g., a type of cancer. Shielding materials can be conjugated to the surface of the lipid bilayer to reduce undesired non-specific binding.

主权项

1. A protocell comprising:
a porous silica nanoparticle core comprising a plurality of pores, said nanoparticle core being formed from an aqueous precursor mixture comprising tetraethylorthosilicate (TEOS) and an amine-modified silane precursor, said porous silica nanoparticle core having an average particle size ranging from about 30 nm to about 3000 nm; and a supported lipid bilayer encasing the porous particle core and supported by the porous particle core, wherein said lipid bilayer consists essentially of a zwitterionic phosphoholipid selected from the group consisting of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), dipalmitoylphosphatidylcholine (DPPC) and a mixture thereof, and wherein said protocell, after administration to a subject, decreases non-specific binding of said protocells in said subject when compared to protocells which are encased with a lipid bilayer comprising positively or negatively charged phospholipids.