Chimeric promoters and methods of use

摘要

Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.

主权项

1. A chimeric promoter construct comprising
a first polynucleotide comprising a regulatory region of a 4-hydroxyphenylpyruvate dioxygenase (HPPD) promoter operably linked to a second polynucleotide comprising a heterologous core promoter functional in a plant, wherein said regulatory region of the HPPD promoter comprises a) the polynucleotide set forth in any one of SEQ ID NO: 2, 3, 4, 11 or 20; b) a polynucleotide comprising a fragment comprising at least 300 consecutive nucleotides of SEQ ID NO: 2, 3, 4, 11, or 20; c) the polynucleotide set forth in any one of SEQ ID NO: 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, or 19; or d) a polynucleotide comprising a fragment comprising at least 300 consecutive nucleotides of SEQ ID NO: 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, or 19; wherein said core promoter modulates the regulatory activity of said regulatory region of the HPPD promoter when compared to the regulatory activity of said regulatory region of the HPPD promoter alone; wherein said chimeric promoter has transcriptional regulatory activity in a plant.