| 摘要 |
Disclosed is wheat grain (Triticum aestivum) comprising an embryo, an endosperm, starch and a reduced level or activity of total starch branching enzyme II (SBEII) protein, wherein the embryo comprises a loss of function mutation in each of 5 to 12 alleles of endogenous SBEII genes selected from the group consisting of SBEIIa-A, SBEIIa-B, SBEIIa-D, SBEIIb-A, SBEIIb-B and SBEIIb-D, such that the level or activity of total SBEII protein in the grain is between 2% and 30% of the level or activity of total SBEII protein in a wild-type wheat grain, said 5 to 12 alleles including 4, 5 or 6 SBEIIa alleles each comprising a loss of function mutation, wherein when the number of SBEIIa alleles comprising a loss of function mutation is only 4 then the number of SBEIIb alleles comprising a loss of function mutation is 6, and wherein the grain comprises an amylose content of at least 50% (w/w) as a proportion of the total starch of the grain. |
