发明名称 |
Methods and Compositions for Size-Controlled Homopolymer Tailing of Substrate Polynucleotides by a Nucleic Acid Polymerase |
摘要 |
The present invention is directed to methods and compositions for adding tails of specific lengths to a substrate polynucleotide. The invention also contemplates methods and compositions for immobilization of tailed substrates to a solid support. The disclosure contemplates that the attenuator molecule is any biomolecule that associates with a tail sequence added to a substrate polynucleotide and controls the addition of a tail sequence to the 3′ end of the substrate polynucleotide. The sequence that is added to the substrate polynucleotide is referred to herein as a tail sequence, or simply a tail, and the process of adding a nucleotide to a substrate polynucleotide is referred to herein as tailing. |
申请公布号 |
US2015087027(A1) |
申请公布日期 |
2015.03.26 |
申请号 |
US201314384113 |
申请日期 |
2013.03.13 |
申请人 |
SWIFT BIOSCIENCES, INC. |
发明人 |
Makarov Vladimir;Kurihara Laurie |
分类号 |
C12P19/34;C12N9/12 |
主分类号 |
C12P19/34 |
代理机构 |
|
代理人 |
|
主权项 |
1. A composition comprising a nucleic acid polymerase and an attenuator molecule. |
地址 |
Ann Arbor MI US |