MICROFLUIDIC REACTORS FOR OLIGONUCLEOTIDE SYNTHESIS

摘要

The present disclosure generally pertains to systems and methods for the chemical synthesis of micro-quantities of oligonucleotides or other chemical molecules. The system includes a reusable glass micro-reactor containing a paramagnetic solid support, a magnet, an electronic drive controller and an optical spectroscopy system capable of driving a plurality individual reactors. The system utilizes the electroosmotic movement of reactants through microfluidic channels. Spectrophotometric monitoring of the reaction products allows for the real-time determination of synthesis yield.

主权项

1. An apparatus for synthesizing a molecule, comprising:
a reaction substrate having at least one reaction chamber and at least one microfluidic channel for transferring a reagent to the reaction chamber; a reagent cassette coupled to the substrate, the reagent cassette having at least one reagent port for transferring the reagent from the cassette to the microfluidic channel; a paramagnetic solid support positioned within the reaction chamber; and a magnet positioned such that the paramagnetic solid support is located within a magnetic field generated in the magnet, wherein the magnetic field is sufficient for holding the paramagnetic solid support in the reaction chamber while the reagent is flowing through the reaction chamber.