| 摘要 |
A method (M1) for the production of synthetic nucleic acids (I), especially double-strand nucleic acids, by (a) making many different nucleic acid fragments by solid-phase synthesis, preferably at different points on a common solid support and (b) combining at least two of these fragments together, in which at least some of the fragments are used in larger amounts than others in stage (b) so as to modulate their thermodynamic parameters. Independent claims are also included for (1) a method (M2) for the production of (I) as above, in which at least some fragments with a high AT content are used in larger amounts than other fragments in stage (b) (2) a method (M3) for the production of (I) as above, which also includes amplification stages in which the synthesised fragments and/or (optionally double-stranded) intermediates formed therefrom are subjected to amplification, e.g. PCR (3) a method (M4) for determining the sequence of a polypeptide (II) with defined properties by making (I) with the corresponding sequence using method M1 followed by (c) using the target sequence for making the polypeptide (II) and (d) determining the properties of (II) (4) polypeptides with defined properties (II) for use in pharmaceutically-active substances and/or in the production thereof, obtained by synthesis of synthetic nucleic acids with the sequence coding for (II) as in method M1, followed by (c) production of (II) using this sequence (5) a method (M5) for the production of (II) comprising stages (a), (b) and (c) . |
