ACTIVE INLET TEMPERATURE CONTROL FOR ADJUSTMENT OF RETENTION IN A LIQUID CHROMATOGRAPHY SYSTEM

摘要

Described are methods in which the retention times of a chromatographic column are adjusted through the control of the temperature of a mobile phase at the inlet to the chromatographic column. The temperature of the mobile phase at the inlet is different from the temperature of the chromatographic column. Adjustment of the temperature of the mobile phase at the inlet can be used as part of a method to transfer a chromatographic method from one liquid chromatography system to another liquid chromatography system. The method can alternatively be adapted for trapping a sample at the head of a chromatographic column to reduce the amount of band broadening of the sample from the sample injector of a liquid chromatography system. A gradient elution can then be performed to cause the concentrated volume of the analyte in the sample to elute in a tight band, resulting in improved measurement sensitivity.

主权项

1. A method for transferring a chromatographic method between liquid chromatography systems, the method comprising:
controlling a temperature of a column thermal zone in a first liquid chromatography system based on a temperature of a column thermal zone in a second liquid chromatography system for a chromatographic method being transferred from the second liquid chromatography system to the first liquid chromatography system, the column thermal zones for the first and second liquid chromatography systems each configured to control a temperature of a chromatographic column therein; and controlling a temperature of a column inlet thermal zone of the first liquid chromatography system to modify a temperature of a mobile phase passing through an inlet to the chromatographic column of the first liquid chromatography system, wherein the temperature of the column inlet thermal zone is set to a temperature that is different from the temperature of the column thermal zone of the first liquid chromatography system, the modification of the temperature of the mobile phase changing the retention times of the chromatographic column in the first liquid chromatography system to be equivalent to the retention times of the chromatographic column in the second liquid chromatography system.