PHOTODAMAGE MITIGATION COMPOUNDS AND SYSTEMS

摘要

Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

主权项

1. A method for protecting an enzyme from photo-induced damage in an illuminated reaction, the method comprising:
providing a reaction mixture comprising the enzyme, and a fluorescent or fluorogenic substrate for the enzyme, wherein interaction of the enzyme and the fluorescent or fluorogenic substrate under excitation illumination results in altered activity of the enzyme; adding a photoprotective agent to the reaction mixture, which photoprotective agent comprises a reducing functional group covalently bound to an oxidizing functional group, and illuminating the reaction mixture with an excitation illumination, wherein the photoprotective agent reduces an amount of photo-induced damage to the enzyme resulting from interaction of the enzyme with the fluorescent or fluorogenic substrate under the excitation illumination to an amount that is less than that which would occur in the absence of the photoprotective agent.