High molecular weight heparosan polymers and methods of production and use thereof

摘要

High molecular weight heparosan polymers are described, as are methods of producing and using the high molecular weight heparosan polymers.

主权项

1. A method for recombinantly producing a high molecular weight heparosan polymer, the method comprising the steps of:
culturing a recombinant host cell having UDP-sugar and transport infrastructure and containing a heterologous nucleotide sequence encoding a Pasteurella heparosan synthase under conditions appropriate for the expression of the heparosan synthase; and isolating heparosan polymers produced by the heparosan synthase, wherein the isolated heparosan polymer is represented by the structure (-GlcUA-beta 1,4-GlcNAc-alpha-1,4-)n, wherein n is a positive integer greater than or equal to 2,000.