Plant extract, compositions containing same, method of extraction and uses thereof

摘要

An extract from leaves of mulberry is disclosed. The extract has an IC50 value sufficient to inhibit α-glucosidase I. The extract may comprise 5-40%(w/w) total imino sugars and 20-70%(w/w) total amino acids. The extract may reduce the production of melanin for the treatment of such ailments or diseases caused by pigmentation as freckle, chloasma, striae gravidarum, sensile plaque and melanoma. The extract may also control blood glucose level.

主权项

1. A plant extract obtained from Morus plant leaves which has an IC50 value of less than 90 μg/ml with respect to inhibition of α-glucosidase I, comprising:
5-40% (w/w) total imino sugars, as measured by at least one of quantitative HPLC High Performance Liquid Chromatography (HPLC) and liquid chromatography/mass spectrometry (LC-MS), including at least one of 1-deoxynojirimycin (DNJ), fagomine, and N-methyl-DNJ; and 20-70% (w/w) total amino acids, wherein the extract is pale yellow and nearly white and is easily soluble in water; wherein the steps of preparing the plant extract comprise: a. subjecting Morus plant leaf material to a water or alcoholic extraction step to obtain a crude extract; b. subjecting the crude extract to a column chromatography purification step using a strong acidic cation exchange resin, washing the column with water, eluting the column with an ammonia solution, then collecting the eluent and removing ammonia therefrom.